Table 2 of
Hanna, Mol Vis 2006;
12:961-976.
Table 2. Primer sets against human sequences used in fixed-slice PCR
The primers were used to detect genes from mRNA extracted from fixed retinal slices. The primers used to detect the glutamate transporters (EAAT1through EATT5) are the same used in fresh retinal tissue and are listed in Table 3. "Accession number" represents GenBank accession number for the corresponding human gene. "Location" represents position of initial nucleotide for forward and reverse primer pairs. "Human sequence" represents sequence for design of forward and reverse primers. "Size" represents number of base pairs in expected PCR product.
Accession Size Gene number Location Human sequence (5'-3') (bp) ------ --------- -------- --------------------------- ---- GluR1 NM_000827 2071 F: TGGTGTCTCCCATTGAGAGTGC 188 2258 R: AATCATCCCCTCCTCTGTGGTC GluR2 NM_000826 1559 F: TTTTTGCCTACATTGGGGTCAG 184 1742 R: TAGTCTCTGGTTTTCCTTGGGTGC GluR3 NM_000828 2072 F: CAGACTGAAATTGCATATGGGACC 191 2262 R: TCCAGCAGGAAGGCAAACTTTC GluR4 NM_000829 2068 F: TGAAAAGATGTGGACCTACATGCG 186 2253 R: GAATCCAGATTTCCTCCCACTTTC GluR5 NM_000830 2428 F: TCTGGCTGCCTTCTTGACAGTAG 204 2631 R: TCACTGTTTCTTACCAGGGCGG GluR6 NM_021956 1977 F: GACAGTGGAACGCATGGAATCC 206 2182 R: CTCGCTGGATTCCTTCTTCATTAC GluR7 NM_000831 2153 F: CTGATGACCTGGCCAAGCAAAC 232 2384 R: TTCCTCTGCGTGACGTACTCGATG KA1 NM_014619 2218 F: GGGAATCGCCAGGGTGTTGAATCC 216 2433 R: TTCAGGATCTCCAGGCGGTTGTTC KA2 NM_002088 2194 F: CTCAACTGCAACCTCACCCAGATC 205 2398 R: CCAAACCTTTAGCTCGATGGTCC mGluR1 NM_000838 2900 F: AATGGCAAGTCTGTGTCATGGTC 186 3085 R: GCTGGTATCTGAAAAGGTCAGGC mGluR2 NM_000839 2043 F: CCATCTGCCTGGCACTTATCTC 199 2241 R: AAGGCATAAAGCGTGCAGAGC mGluR3 NM_000840 2047 F: ATCAAGCACAACAACACACCCTTG 186 2232 R: CAGCAGGGCTGAGTAACAGATAGC mGluR4 NM_000841 2683 F: ACATGCCCAAAGTCTACATCATCC 199 2881 R: TAAGTCTGTTTGGTGGCCAGCG mGluR5 NM_000842 2019 F: TGGCATCTGCCTGGGCTACTTATG 205 2223 R: ACAGGCACTCATGCCTCTGGGC mGluR6 NM_000843 2302 F: AGGTGGTGGGGATGATAGCATG 190 2491 R: TTGATGGCGTACACTGTGCACG mGluR7 NM_000844 2005 F: CTGGGCTGTGATTCCTGTCTTC 225 2229 R: AAGCCCAAGAAAACTCGCCG mGluR8 NM_000845 2001 F: CTCCTTCCGACGGGTCTTCCTA 181 2181 R: AAGGAGCTGGACGGAGATGA |