Table 2 of
Hanna, Mol Vis 2006;
12:961-976.Table 2 of
Hanna, Mol Vis 2006;
12:961-976.
Table 2. Primer sets against human sequences used in fixed-slice PCR
The primers were used to detect genes from mRNA extracted from fixed retinal slices. The primers used to detect the glutamate transporters (EAAT1through EATT5) are the same used in fresh retinal tissue and are listed in Table 3. "Accession number" represents GenBank accession number for the corresponding human gene. "Location" represents position of initial nucleotide for forward and reverse primer pairs. "Human sequence" represents sequence for design of forward and reverse primers. "Size" represents number of base pairs in expected PCR product.
Accession Size
Gene number Location Human sequence (5'-3') (bp)
------ --------- -------- --------------------------- ----
GluR1 NM_000827 2071 F: TGGTGTCTCCCATTGAGAGTGC 188
2258 R: AATCATCCCCTCCTCTGTGGTC
GluR2 NM_000826 1559 F: TTTTTGCCTACATTGGGGTCAG 184
1742 R: TAGTCTCTGGTTTTCCTTGGGTGC
GluR3 NM_000828 2072 F: CAGACTGAAATTGCATATGGGACC 191
2262 R: TCCAGCAGGAAGGCAAACTTTC
GluR4 NM_000829 2068 F: TGAAAAGATGTGGACCTACATGCG 186
2253 R: GAATCCAGATTTCCTCCCACTTTC
GluR5 NM_000830 2428 F: TCTGGCTGCCTTCTTGACAGTAG 204
2631 R: TCACTGTTTCTTACCAGGGCGG
GluR6 NM_021956 1977 F: GACAGTGGAACGCATGGAATCC 206
2182 R: CTCGCTGGATTCCTTCTTCATTAC
GluR7 NM_000831 2153 F: CTGATGACCTGGCCAAGCAAAC 232
2384 R: TTCCTCTGCGTGACGTACTCGATG
KA1 NM_014619 2218 F: GGGAATCGCCAGGGTGTTGAATCC 216
2433 R: TTCAGGATCTCCAGGCGGTTGTTC
KA2 NM_002088 2194 F: CTCAACTGCAACCTCACCCAGATC 205
2398 R: CCAAACCTTTAGCTCGATGGTCC
mGluR1 NM_000838 2900 F: AATGGCAAGTCTGTGTCATGGTC 186
3085 R: GCTGGTATCTGAAAAGGTCAGGC
mGluR2 NM_000839 2043 F: CCATCTGCCTGGCACTTATCTC 199
2241 R: AAGGCATAAAGCGTGCAGAGC
mGluR3 NM_000840 2047 F: ATCAAGCACAACAACACACCCTTG 186
2232 R: CAGCAGGGCTGAGTAACAGATAGC
mGluR4 NM_000841 2683 F: ACATGCCCAAAGTCTACATCATCC 199
2881 R: TAAGTCTGTTTGGTGGCCAGCG
mGluR5 NM_000842 2019 F: TGGCATCTGCCTGGGCTACTTATG 205
2223 R: ACAGGCACTCATGCCTCTGGGC
mGluR6 NM_000843 2302 F: AGGTGGTGGGGATGATAGCATG 190
2491 R: TTGATGGCGTACACTGTGCACG
mGluR7 NM_000844 2005 F: CTGGGCTGTGATTCCTGTCTTC 225
2229 R: AAGCCCAAGAAAACTCGCCG
mGluR8 NM_000845 2001 F: CTCCTTCCGACGGGTCTTCCTA 181
2181 R: AAGGAGCTGGACGGAGATGA
|