Figure 5.

Overexpression of K6W-ubiquitin inhibits the degradation of p27^KIP. HLEC were synchronized and infected with adenovirus as described in Figure 3. After replating, the cells were collected at the indicated times. Levels of p27^KIP were determined by western blotting as in Figure 4. The western blot (A) and a densitometry quantification (B) after normalization of protein loading as described in Figure 4 are shown. Experiments were performed three times with reproducible results. The asterisk indicates a p<0.01 as comparing cells infected with adenovirus encoding K6W-ubiquitin with cells infected with empty adenovirus.