Table 3 of Rex, Mol Vis 2005; 11:1236-1245.


Table 3. Properties of electroretinograms of mice with genomic delivery of enhanced green fluorescent protein

Column 1 identifies the promoter driving enhanced green fluorescent protein expression in an individual transgenic animal. All animals were between six and eight weeks of age at the time of electroretinogram (ERG) testing. Columns 2-6 of the first row identify specific parameters measured from the ERG (see Figure 4): bmax (rods), the saturating amplitude of the scotopic b-wave (Figure 4A); Sb represents the sensitivity of the scotopic b-wave expressed as fraction of the normalized response per photoisomerization; amax is the saturating amplitude of the a-wave and A is the amplification coefficient (with dimensions s-2), estimated from a set of a-waves (Figure 4B); bmax (cones) is the saturating amplitude of the cone-driven b-wave. "ND" signifies "not determined." The final row of the table gives average parameters from a population of C57Bl/6 mice of 8 wks age [34] (Table 2). Asterisk indicates that the ERG experiments in [34] were performed with the reference electrode inserted into the animals forehead, while for those reported here the reference electrode was in the mouth. The amplitudes for bmax and amax of the WT population data have been multiplied by the experimentally determined scale factor 1.9 to account for the change in reference electrode placement.

                       bmax                                      bmax
              Age     (rods;                 amax               (cones;
Promoter    (weeks)     μV)     Sb (Δβ/Φ)    (μV)        A        μV)
---------   -------   -------   ---------   -------   -------   -------
β-actin        8        120       0.24        200       2.6       100
β-actin        5        350       0.48        365       2.0       225
β-actin        5        470       0.13        420       3.3       200
β-actin        5        550       0.16        370       7.5       200
LMHCops        7        140       0.25        225       2.8        60
LMHCops        7        245       ND          320       ND         75
LMHCops        7        370       0.24        400       8.0        86
LMHCops        7        410       0.14        390       9.8        95
LMHCops        7        475       0.22        323       3.6        65

WT             8      415±140   0.28±0.04   550±125   7.4±2.5   210±55
controls*
(Mean±SD;
n=56)

Rex, Mol Vis 2005; 11:1236-1245 <http://www.molvis.org/molvis/v11/a141/>
©2005 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535