Figure 3 of Steele, Mol Vis 2005; 11:1200-1210.


Figure 3. Fluoxetine suppresses elevation of intracellular calcium levels in salamander rod photoreceptors following depolarization

Fluoxetine (10 μM) in the bath virtually eliminates the normal elevation of intracellular calcium levels in both somatic and terminal compartments of salamander rod photoreceptors following brief depolarization with 100 mM K+. A: A representative fluorescent (380 nm excitation, 510 nm emission) image of an isolated salamander rod photoreceptor with intact axon and presynaptic terminal loaded with fura-2. Changes in calcium dynamics were measured as changes in fluorescence within regions of interest (outlined with hatched circular lines) corresponding to the terminal (1) and somatic (2) compartments of the cell for comparison. The scale bar represents 10 μm. B: Representative trace of changes in intracellular calcium levels within the presynaptic terminal (1) and somatic (2) compartments are plotted as changes in 340/380 nm ratios over time following a brief depolarizing puff (indicated by the arrow) of 100 mM K+ (pipette concentration) in control bath solution or bath solution containing 10 μM fluoxetine for the same cell. C: Statistical comparison of changes in calcium, shown as changes in 340/380 nm fluorescence ratio over time (in seconds), of somatic and terminal compartments of cells (n=7) stimulated first in the presence of control bath solution and then in bath solution containing 10 μM fluoxetine. Note that 10 μM fluoxetine in the bath virtually abolishes the depolarization-induced elevation of intracellular calcium in both somatic and terminal compartments of all cells. Statistical tests were done using Student's t-test.

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Steele, Mol Vis 2005; 11:1200-1210 <http://www.molvis.org/molvis/v11/a137/>
©2005 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535