Figure 1 of Steele, Mol Vis 2005; 11:1200-1210.


Figure 1. Test puffs of fluoxetine hydrochloride inhibit ICl(Ca) tail currents and outward currents of isolated salamander rod photoreceptors induced by depolarization in a dose-dependent fashion

A: Enzymatically dissociated salamander rod photoreceptors possess intact rhodopsin-containing outer segment (OS), soma (S), axon (A), and presynaptic terminals (T). The scale bar represents 10 μm. B: Representative current responses from intact dissociated salamander rods resulting from a 2 s depolarizing voltage step from -60 mV to 0 mV were recorded from the same salamander rod photoreceptor before (1), during (2), and after washout (3) of a test puff of medium containing 50 μM fluoxetine hydrochloride, 10 μM fluoxetine hydrochloride, or drug vehicle (DMSO) only. Traces shown for each concentration of fluoxetine were recorded from different cells. Note the virtual elimination of both the ICl(Ca) tail current and outward currents by 50 μM fluoxetine. A test puff of 10 μM fluoxetine results in less significant changes in the initial amplitude of both outward and ICl(Ca) tail currents but significantly shortens the duration of the sustained ICl(Ca) tail current. DMSO alone did not alter the control currents. C: Statistical comparison of ICl(Ca) tail currents and outward currents measured in the presence of a 50 μM fluoxetine test puff (n=9) or a 10 μM fluoxetine test puff (n=5) with currents measured from the same cells in control bath solution. The control currents for each cell were defined as 1 for normalization purposes. Also shown is a comparison of the duration of ICl(Ca) tail currents measured in the presence of a 10 μM fluoxetine test puff with those measured from the same cells in control bath solution. The control duration for each cell was defined as 1 for normalization purposes.

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Steele, Mol Vis 2005; 11:1200-1210 <http://www.molvis.org/molvis/v11/a137/>
©2005 Molecular Vision <http://www.molvis.org/molvis/>
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