Table 2 of
Ohno-Matsui, Mol Vis 2005;
11:1-10.Table 2 of
Ohno-Matsui, Mol Vis 2005;
11:1-10.
Table 2. Primer sequences used for real-time quantitative PCR
Some of the primers listed in Table 1 were used for the detection of tyrosinase, cathepsin D, bFGF, BDNF, PEDF, and FGFR.
Product
Primer sequence (5'-3') Annealing size Accession
Gene position in mRNA temp (°C) (bp) number
---------- -------------------------- --------- ------- ---------
RPE65 F: CCTTTCTTCATGGAGTCTTTG 50 122 BC075035
R: ATTGCAGTGGCAGTTGTATTG
CRALBP F: GCTGCTGGAGAATGAGGAAACT 61 149 BC004199
R: TGAACCGGGCTGGGAAGGAATC
TRP2 F: GGTTCCTTTCTTTCCAGT 60 185 S69231
R: GAAGAAAAGCCAACAGCACAA
bestrophin F: ATGGGGCCTTGATGGSAGCAC 58 227 BC015220
R: GGCGAAGCATCCCCATTAGG
GAPDH F: TGAACGGAAGCTCACTGG 65 307 M33197
R: TCCACCACCCTGTTGCTGTA
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