Table 1 of
Ohno-Matsui, Mol Vis 2005;
11:1-10.Table 1 of
Ohno-Matsui, Mol Vis 2005;
11:1-10.
Table 1. Primer sequences used for semi-quantitative PCR
This table describes the primer sequences used in RT-PCR. The results of RT-PCR are shown in Figure 4.
Product
Primer sequence (5'-3') Annealing size
Gene position in mRNA temp (°C) (bp) Reference
----------- ----------------------------------- --------- ------- ---------
RPE65 F: CCTTTCTTCATGGAGTCTTTG 52 390 [57]
R: ATTGCAGTGGCAGTTGTATTG
CRALBP F: ATGTCAGAAGGGGTGGG 60 953 [57]
R: TCAGAAGGCTGTGTTCTCA
TRP2 F: GGAGAAAAGTACGACAGAGACAAGG 60 1542 [25]
R: AGAAAAGCCAACAGCACAAAAAGAC
tyrosinase F: GCATCATTCTTCTCCTCTTGG 60 359 [25]
R: ATAGGATCGTTGGCAGATCC
cathepsin D F: GTGCTTCACAGTCGTCTTC 55 172 [58]
R: GAGCCATAGTGGATGTCAAAC
bFGF F: GCCTTCCCGCCCGGCCACTTCAAGG 55 179 [57]
R: GCACACACTCCTTTGATAGACACAA
BDNF F: ATGACCATCCTTTTCCTTACTATGGT 52 741 [57]
R: TCTTCCCCTTTTAATGGTCAATGTAC
PEDF F: GGACGCTGGATTAGAAGGCAG 64 675 [39]
R: TTGTATGCATTGAAACCTTACAGG
FGFR F: GGGTCCATCAATCACACGTAC 60 643 [21]
R: GGCGTGTTGTTATCCTCACCAG
bestrophin F: GGCCAGATCTATGTACTGGAATAAGCCCGAGC 65 773 [36]
R: GGCCCTCGAGTTAGGAATGTGCTTCATCCCTG
β-actin F: GAGCACAGAGCCTCGCCTTTGC 65 620 [57]
R: GGATCTTCATGAGGTAGTCAGTCAGG
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