Figure 5. DNAse I hypersensitivity and ChIP analyses of the region between OGN/mimecan and OMD genes

DNAse I hypersensitivity and ChIP analyses of the region between OGN/mimecan and OMD genes on human chromosome 9. A: Schematic of the region between the mimecan and OMD genes on human chromosome 9 and positions of the HOX-Runx model matches. Binding sites for factors that have been characterized previously also are indicated. These are; the E-box (binding site for USF1), ISRE (binding site for IRF1 and IRF2), and p53 (binding site for tumor suppressor protein p53) [45-48]. B: DNAse I hypersensitivity PCR analysis. Cells were treated with the indicated amount of DNAse I, or incubated with buffer lacking DNAse I (input), lysed, and genomic DNA was isolated and used as template for PCR with indicated primers (Table 2). C: ChIP analysis. Chromatin immunoprecipitation was performed using indicated cells and antibodies. PCR of input DNA shows equivalent starting material for the assay.