Table 1 of
Mansfield, Mol Vis 2004;
10:521-532.Table 1 of
Mansfield, Mol Vis 2004;
10:521-532.
Table 1. Comparison of effects of FGF and other growth factors on TGFβ treated lens epithelial explants
Explants were cultured with 50 pg/ml TGFβ for one day then, after replacing medium, with FGF, EGF, PDGF, IGF, or HGF, at the concentrations indicated in the legend of Figure 3, or without further addition. Controls received no growth factors throughout the culture period. Four categories of response were assessed: wrinkling of the lens capsule, spindle cell formation, cell surface blebbing, and cell loss. The number of pluses indicates the proportion of the explant exhibiting a particular feature. A dash indicates no change. An asterisk indicates the feature could not be assessed due to extensive cell loss. Explants that were initially poorly covered with cells (10% or less) were excluded from this assessment. Throughout the culture period, control explants remained in cobblestone arrays typical of the normal lens epithelium, whereas cells in the other groups, although still monolayered, were often elongated and irregularly arrayed. Prior to day 3 the only changes noted were a slight wrinkling in the central region and scattered spindle cell formation in some explants in the TGFβ and TGFβ plus EGF groups. By day 9, all explants in the TGFβ and the TGFβ plus EGF, TGFβ plus PDGF, TGFβ plus IGF, and TGFβ plus HGF groups were virtually devoid of cells.
TGFβ TGFβ TGFβ TGFβ TGFβ
Response Control TGFβ +FGF +EGF +PDGF +IGF +HGF
------------- ------- ----- ---- ----- ----- ----- -----
Day 3
Wrinkling - ++++ - +++ +++ ++ ++++
Spindle cells - ++ -/+ ++ ++ +++ ++
Blebbing - ++++ - ++++ ++++ ++++ ++++
Cell loss - + -/+ + + + +
Day 4
Wrinkling - ++++ - ++++ ++++ ++ ++++
Spindle cells - * ++ * * * *
Blebbing - +++++ + +++++ +++++ +++++ +++++
Cell loss - +++ -/+ +++ +++ +++ +++
Day 6
Blebbing -/+ * + +++++ * * *
Cell loss -/+ +++++ + +++++ ++++ ++++ +++++
Number of
explants 4 6 8 4 3 5 6
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