Table 8 of
Harrington, Mol Vis 2004;
10:476-489.Table 8 of
Harrington, Mol Vis 2004;
10:476-489.
Table 8. Post-translational modifications identified in HMW and WI proteins of cataractous lenses
Identification of post-translational modifications in HMW and WI proteins of cataractous lenses. The 2D gel electrophoretically separated protein spots of WS-HMW proteins (Figure 5B) and WI proteins (Figure 7) from cataractous lenses were examined using the MALDI-TOF mass spectrometric method. The oxidation or deamidation of specific residues in tryptic fragments was identified based on the changes in the mass of the fragments.
Spot Crystallin
number fragment Modification(s)
------ ---------- -----------------------
HMW Proteins
1 αB Oxidation (W60)
Deamidation (N146)
2 αB Oxidation (W60)
Deamidation (N146)
3 αB Oxidation (W60)
Deamidation (N146)
4 αB Deamidation (N146)
5 βB1 Deamidation βA3/A1
βA3/A1 (N54, N62)
6 βB1 Deamidation βA3/A1
βA3/A1 (N54, N62, N108)
7 βB1 Deamidation (N216)
8 βB1 Deamidation
(N107, N161)
WI Proteins
2 αB Oxidation (W60)
Deamidation (N78, N146)
3 αB Oxidation (W60)
Deamidation (N146)
4 αB Oxidation (W60)
Deamidation (N146)
5 αA -
6 αA -
7 αB Oxidation (W60)
Deamidation (N78)
8 αB Oxidation (W60)
Deamidation (N78, N146)
9 αB Deamidation (N78, N146)
10 αB Oxidation (W60)
Deamidation (N78, N146)
11 αB Oxidation (W60)
Deamidation (N146)
12 αB Deamidation (N146)
13 αB Oxidation (W60)
Deamidation (N78, N146)
14 αB Deamidation (N146)
15 αB Oxidation (W60)
Deamidation (N78, N146)
16 αB Deamidation (N146)
17 αB Oxidation (W60)
Deamidation (N146)
18 αB Oxidation (W60)
Deamidation (N78, N146)
19 αB Deamidation (N78, N146)
20 αB -
21 αB -
22 βA3/A1 Deamidation (N133)
23 βA3/A1 -
24 βA3/A1 -
25 βA3/A1 Deamidation (N40, N133)
26 βA3/A1 Oxidation (W96)
Deamidation (N40, N133)
27 βB1 -
28 βA3/A1 Deamidation (N40)
29 βA3/A1 Deamidation (N40)
30 βA3/A1 Deamidation (N40)
31 βA3/A1 Deamidation (N40)
32 βA3/A1 Deamidation (N40)
33 βA3/A1 Deamidation (N40)
34 βB1 -
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