Table 7 of
Harrington, Mol Vis 2004;
10:476-489.Table 7 of
Harrington, Mol Vis 2004;
10:476-489.
Table 7. Parent crystallins of spots present in a 2D gel of WI proteins from cataractous lenses
Identification of parent crystallins of spots present in the 2D gel of WI protein fractions of cataractous lenses with nuclear opacity from 50-60 year old donors (Figure 7) by tryptic peptide fingerprinting mass spectrometry. Molecular weights were determined based on migration of protein markers. "UD" indicates undetermined. In the cleavage column, "C" indicates C-terminus cleavage and "N" indicates N-terminus cleavage.
Molecular Percent
Spot Parent weight sequence
number crystallin Cleavage (kDa) detected
------ ---------- -------- --------- --------
1 UD UD 14.7 UD
2 αB UD 15.0 UD
3 αB N 16.1 58
4 αB N and C 16.3 55
5 αA C 18.9 40
6 αA N and C 19.5 24
7 αB N 18.9 37
8 αB N 19.5 62
9 αB Intact 20.3 100
10 αB N 19.9 39
11 αB UD 20.3 UD
12 αB UD 20.3 UD
13 αB UD 20.3 UD
14 αB Intact 20.7 100
15 αB Intact 21.2 100
16 αB Intact 21.2 100
17 αB N 21.2 63
18 αB UD 20.7 UD
19 αB UD 21.4 UD
20 αB UD 20.7 UD
21 αB UD 22.0 UD
22 βA3/A1 N 22.7 55
23 βA3/A1 N 22.7 32
24 βA3/A1 N 22.7 42
25 βA3/A1 UD 22.7 UD
26 βA3/A1 N 22.7 37
27 βB1 Intact 22.7 100
28 βA3/A1 C 25.4 30
29 βA3/A1 N 26.8 23
30 βA3/A1 Intact 25.4 100
31 βA3/A1 N and C 25.4 20
32 βA3/A1 C 25.4 20
33 βA3/A1 C 25.4 12
34 βA3/A1 N 26.2 UD
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