Table 4 of
Harrington, Mol Vis 2004;
10:476-489.Table 4 of
Harrington, Mol Vis 2004;
10:476-489.
Table 4. Parent crystallins of spots in a 2D gel of WS-HMW proteins from normal lenses
Identification of spots present in 2D gel of HMW protein fraction isolated from normal lenses. Spot number corresponds to the number shown in Figure 5. In the cleavage column, "C" indicates C-terminus cleavage and "N" indicates N-terminus cleavage. Molecular weights were determined based on migration of protein markers.
Molecular
Spot Parent weight
number crystallin Cleavage (kDa)
------ ---------- ------------------ ---------
1 αA N and C 2.5
2 αB N and C 2.9
3 αB N and C 3.6
4 αA N and C
αB N 3.9
5 αA N and C 4.5
6 αA N and C 4.5
7 αA N and C 4.9
8 αB N and C 5.1
9 αA N 5.5
10 αA N and C 5.9
11 αA N and C 6.0
12 αB N and C 6.0
13 αB C 6.3
14 αB N and C 7.2
15 αB N and C 7.4
16 αA N and C 8.85
17 αA N and C 9.6
18 αB N and C 9.6
19 αA N and C 10.5
20 αB C 12.5
21 αB N and C 13.5
22 αA N 10.5
23 αB N 14.5
24 αB N 14.5
25 αB Mixture of both 18.0
N and C
terminally cleaved
26 αB N and C 19.0
27 αB N 18.0
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