Table 3 of Harrington, Mol Vis 2004; 10:476-489.


Table 3. Parent crystallins of spots in a 2D gel of WS-α-crystallin fraction from 60 year old cataractous lenses

Identification of parent crystallins of spots present in 2D gels of the WS crystallin fraction of cataractous lenses with nuclear opacity Spot number corresponds to numbers shown in Figure 4. In the cleavage column, "C" indicates C-terminus cleavage and "N" indicates N-terminus cleavage. Molecular weights were determined based on migration of protein markers.

                                 Molecular
 Spot      Parent                 weight
number   crystallin   Cleavage     (kDa)
------   ----------   --------   ---------
   1         αA       N and C       3.1
   2         αA       N and C       3.7
   3         αB       N and C       6.1
   4         αA       N and C       6.1
   5         αA       N and C       5.0
   6         αB       N and C       9.0
   7         αA       N and C       9.5
   8         αA          N          9.0
   9         αA       N and C      15.2
  10         αA       N and C      15.2
  11         αA       N and C      15.3
  12         αA       N and C      15.3
  13     Mixture of   N and C      15.3
         αA and αB
  14         αB       N and C      19.7
  15         αA       N and C      19.5
  16         αB          N         19.2
  17         αB       N and C      19.2
  18         αB        Intact      20.7
  19         αB        Intact      20.7
  20         αA        Intact      20.7
  21         αB        Intact      20.7

Harrington, Mol Vis 2004; 10:476-489 <http://www.molvis.org/molvis/v10/a61/>
©2004 Molecular Vision <http://www.molvis.org/molvis/>
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