Table 3 of
Harrington, Mol Vis 2004;
10:476-489.
Table 3. Parent crystallins of spots in a 2D gel of WS-α-crystallin fraction from 60 year old cataractous lenses
Identification of parent crystallins of spots present in 2D gels of the WS crystallin fraction of cataractous lenses with nuclear opacity Spot number corresponds to numbers shown in Figure 4. In the cleavage column, "C" indicates C-terminus cleavage and "N" indicates N-terminus cleavage. Molecular weights were determined based on migration of protein markers.
Molecular Spot Parent weight number crystallin Cleavage (kDa) ------ ---------- -------- --------- 1 αA N and C 3.1 2 αA N and C 3.7 3 αB N and C 6.1 4 αA N and C 6.1 5 αA N and C 5.0 6 αB N and C 9.0 7 αA N and C 9.5 8 αA N 9.0 9 αA N and C 15.2 10 αA N and C 15.2 11 αA N and C 15.3 12 αA N and C 15.3 13 Mixture of N and C 15.3 αA and αB 14 αB N and C 19.7 15 αA N and C 19.5 16 αB N 19.2 17 αB N and C 19.2 18 αB Intact 20.7 19 αB Intact 20.7 20 αA Intact 20.7 21 αB Intact 20.7 |