Figure 1. Isolation and identification of WS-α-crystallin protein fractions

This figure shows the separation of α-crystallin fractions from the WS proteins of human lenses following size-exclusion chromatography. Size exclusion HPLC using a TSK G-4000 PW_XL column of WS proteins from lenses of 21-26 year old donors. During HPLC, 50 mM Tris-HCl buffer (pH 7.9) was used for both column equilibration and sample elution, and eluted fractions were maintained at 5 °C during HPLC. A: Protein elution profile at 280 nm of WS proteins from 21-26 year old lenses during size exclusion HPLC using a TSK G-4000 PWXL column. B: Identification of α-crystallin containing fractions by SDS-PAGE using a 15% polyacrylamide gel among column fractions. Note that α-crystallin eluted at 3-16 min as shown in A and are represented by fractions 13-16 in B.