Figure 5. Alternative APC transcripts in the RPE and epiretinal membrane

Comparative quantitative RT-PCR in polyacrylamide gel using human exons BS and 3, and cytokeratin-18 primers was used to identify APC isoforms in human RPE cells and from human ERM samples. The positions for exon 1 containing (BS-APC + exon 1) or exon 1 lacking (BS-APC-exon 1) APC transcripts, cytokeratin-18, and internal standard β-actin are indicated for RPE and ERM. Result of ERM represents one of three patients (P2).