Table 3 of
Ohngemach, Mol Vis 2004;
10:1019-1027.
Table 3. Real-time PCR amplification characteristics
The table shows slopes, efficiencies, and correlations of PCR amplifications determined by dilution series; the coefficients of variation (CV) of the mean CT for reference and target genes are also shown.
Gene Slope Efficiency Correlation CV±SD ------------- ----- ---------- ----------- --------- 18S rRNA (1) -3.32 2.05 -1.00 0.82±0.69 18S rRNA (2) -2.83 2.25 -0.98 0.99±0.66 selenoprotein -2.93 2.20 -0.99 0.54±0.35 prolidase -3.24 2.03 -0.99 0.77±0.75 sequence 3 -2.36 2.65 -0.96 0.72±0.38 |