Table 3 of Ohngemach, Mol Vis 2004; 10:1019-1027.


Table 3. Real-time PCR amplification characteristics

The table shows slopes, efficiencies, and correlations of PCR amplifications determined by dilution series; the coefficients of variation (CV) of the mean CT for reference and target genes are also shown.

   Gene         Slope   Efficiency   Correlation     CV±SD
-------------   -----   ----------   -----------   ---------
18S rRNA (1)    -3.32      2.05         -1.00      0.82±0.69

18S rRNA (2)    -2.83      2.25         -0.98      0.99±0.66

selenoprotein   -2.93      2.20         -0.99      0.54±0.35

prolidase       -3.24      2.03         -0.99      0.77±0.75

sequence 3      -2.36      2.65         -0.96      0.72±0.38

Ohngemach, Mol Vis 2004; 10:1019-1027 <http://www.molvis.org/molvis/v10/a121/>
©2004 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535