Table 2 of
Ohngemach, Mol Vis 2004;
10:1019-1027.Table 2 of
Ohngemach, Mol Vis 2004;
10:1019-1027.
Table 2. Sequences of primer pairs
The primer pairs listed in the table were used in the real-time PCR experiments. 18S rRNA (1) served as housekeeping gene for normalization of selenoprotein expression and 18S rRNA (2) for normalization of prolidase and sequence 3 expression. The predicted amplicon size is given.
Forward (F) and Amplicon
Gene reverse (R) primers size (bp)
------------- -------------------------------- ---------
18S rRNA (1) F: 5'-CGGAGAGGGAGCCTGAGAA-3' 275
R: 5'-CGCCAGCTCGATCCCAAGA-3'
18S rRNA (2) F: 5'-GCCCTCCAATGGATCCTCG-3' 174
R: 5'-CGCCAGCTCGATCCCAAGA-3'
selenoprotein F: 5'-AGTCCAGGCTTTTGGTCAGG-3' 164
R: 5'-CCACGCTGTCTGTATTCCAC-3'
prolidase F: 5'-TACGAGCTGGAGAGCCTTTTC-3' 167
R: 5'-ACAGGCACAGGTCTCCATCTTCA-3'
sequence 3 F: 5'-GGAGTGGAGATGAACGAGGC-3' 121
R: 5'-TGGCAAGATCCTTCTTAAAGCTG-3'
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