Figure 1. Expression constructs used for in ovo electroporation experiments

A: In pCAX-EGFP, enhanced green fluorescence protein (EGFP) expression is driven by a chimeric promoter containing a chick β-actin promoter fused to CMV IE enhancer. B: The pMES-cNf2 vector. Chicken Nf2 (cNf2) cDNA was inserted between the EcoRI and SalI sites of the multiple cloning site (MCS) in vector pMES [28]. This plasmid contains the same regulatory element, β-actin promoter/CMV-IE enhancer, as in pCAX (Figure 1A), an internal ribosomal entry site (IRES), and the EGFP gene. In the transfected cells, cNf2 protein product (c-merlin) is co-expressed with EGFP protein from a cNf2-EGFP fusion transcript.