Table 1 of
Burdon, Mol Vis 2003;
9:710-714.Table 1 of
Burdon, Mol Vis 2003;
9:710-714.
Table 1. PCR amplification of albinism genes
Primer sequences for PCR amplification of albinism genes were designed or taken from the literature as indicated. Exon 1 of Tyrosinase gene was amplified in three overlapping fragments. The optimal annealing temperature is given.
Primer sequence 5'-3' Size of PCR Annealing
Gene Exons or reference product temperature
---------- ---------------------------- --------------------- ----------- -----------
Tyrosinase 1A,1B,2-5 [22] 52
1C [22] 65
Enhancer GGCAAGTGTAAGGCAAAATTC 315 58
TTTGAGACAGAACAGGCTTTG
Promoter TACCTCTCATTTGCAAGGTCA 400 58
TCACAGATTTCTCTTTCCAGC
P Gene 2,3,6,7,10,11,14,16-18,21,24 [21] 58
4,9 [21] 65
5 ATGGAAGTTACTCAAGGCTGC 217 60
TATACAGCCAAAGGCACACAG
8,12,13,19,25 [21] 56
15 [21] 60
20 [21] 50
TYRP1 1 (including promoter) CCAAATTAGTGCTTCTGGC 360 60
CTAATGGAGTTTTGGCACG
2-8 [23] 55
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