Figure 11. VAMP-1 does not colocalize with markers for amacrine or bipolar cells and terminals

A-C: Double-labeling for VAMP-1 and GAD-65 (GAD), a marker for GABAergic amacrine cell terminals. VAMP-1 (arrowheads) and GAD-65 (arrows) label different populations of puncta in the IPL. The VAMP-1-immunoreactive plexus in the mid-IPL (square bracket symbol) costratifies with a GAD-65-immunoreactive plexus located between the ON and OFF cholinergic plexes (small arrowheads labeled on and off). Two VAMP-1-immunoreactive ganglion cells (*) are visible in the GCL. Blood vessels (bv) show non-specific labeling. D-F: Double-labeling for VAMP-1 and choline acetyltransferase (CHAT), a marker for the cholinergic starburst amacrine cells (A) and their processes. The VAMP-1-immunoreactive plexus in the mid-IPL (square bracket symbol) is located between the ON and OFF cholinergic plexuses (small arrowheads labeled on and off). Two VAMP-1-immunoreactive cells (*) and their primary dendrites (arrows) are visible in the GCL. G-I: Confocal microscopic image (single optical section, 0.3 μm thickness) of double labeling for VAMP-1 and glycine (GLY), a marker for many amacrine and displaced amacrine cells (A and *, respectively). Similar to GAD-65, VAMP-1 (arrowheads) and GLY (arrows) labels do not colocalize in the IPL. Numerous amacrine cells (A) show strong labeling for GLY in their som A presumptive displaced amacrine cell (*) shows weak labeling for GLY. J-L: Double-labeling for VAMP-1 and VGLUT1, a marker for bipolar cell terminals in the IPL. Puncta in the IPL showing VAMP-1 labeling (arrowheads) do not colocalize with the synaptic terminals of bipolar cells labeled for VGLUT1 (arrows). A large VAMP-1-immunoreactive cell (*) is visible in the GCL. A second, smaller VAMP-1-immunoreactive cell body is visible to its right. Scale bars represent 10 μm.