Figure 10. VAMP-1 does not colocalize with other synaptic vesicle markers in the IPL

A-C: Double-labeling for VAMP-1 and VAMP-2. Punctate labeling for VAMP-1 is present (large arrowheads), particularly in a broad band located in the mid-IPL (identified with brackets). A cell in the ganglion cell layer (*) and its primary dendrite (small arrowhead) shows intense labeling for only VAMP-1. Labeling for VAMP-2 is present in terminals throughout the IPL. Puncta in the IPL show only single labeling for either VAMP-1 (large arrowheads) or for VAMP-2 (arrows). D-F: Double-labeling for VAMP-1 and synapsin I (SYN I) in the IPL. Puncta labeled for VAMP-1 (large arrowheads) are present, including a prominent band in the mid-IPL (identified with brackets). An intensely labeled cell (*) and its dendrites (small arrowheads) also are visible. Labeling for synapsin I is present in numerous conventional synaptic terminals throughout the IPL (arrows), but does not colocalize with VAMP-1 labeling. G-I: Double-labeling for VAMP-1 and synaptic vesicle protein 2 (SV2), a ubiquitous synaptic vesicle marker. Puncta labeled for VAMP-1 (large arrowheads) are present in the IPL, including a prominent band located in the mid-IPL (identified with brackets). Two intensely labeled cells (large *), their dendrites (small arrowheads) and a bundle of axons (ax) are also visible. The cell at the right projects to the VAMP-1 plexus located in the mid-IPL (identified with brackets). Labeling for SV2 is present in numerous synaptic terminals throughout the IPL (arrows), including the large rod bipolar cell terminals along the inner margin of the IPL (small *). The two markers do not colocalize. Scale bars represent 10 μm.