Figure 3 of
Lee, Mol Vis 2003;
Figure 3. Effect of C3 exoenzyme and Y27632 in CECs stimulated with FGF-2
A: First passage cells (1x104) were plated in a 4 well chamber and maintained for 2 days in the presence of 10 ng/ml of FGF-2 in DMEM-10. On day 3, cells were treated with either 10 μM of Y27632 or 5 μg/ml of C3 exoenzyme in the presence of FGF-2 for an additional 24 h. Cells were then fixed with and stained for F-actin (red) and vinculin (green) and merged images are shown in yellow. Negative control was stained in the absence of primary antibody. Bar represents 10 μm. B: First passage cells (1x105) plated in 35 mm dishes were maintained in FGF-2 (10 ng/ml) in DMEM-10. On day 3, 10 μM of Y27632 was added to the cells for a designated time up to 24 h. Data are representative of four experiments. C: First passage cells (1x105) plated in 35 mm dishes were treated with 10 ng/ml of FGF-2 in DMEM-10 for 2 days. On day 3, cells were further treated for 24 h with one of the following experimental conditions; 10 μM of Y27632, 10 μM of Y27632 with 5 mg/ml of neutralizing antibody to FGF-2, or 10 μM of Y27632 with 20 μM of LY294002. All images were magnified 200 times from their original size. Data shown are representative of four experiments.