Table 2 of
Jiang, Mol Vis 2003;
9:579-583.Table 2 of
Jiang, Mol Vis 2003;
9:579-583.
Table 2. Primer pairs used for sequencing
Primer pairs used for sequencing. GJA3 gene contains only one exon. For mutation analysis, four pairs of primers were designed covering the entire exon. The genomic DNA PCR fragments, which are overlapped, were then sequenced using ABI3100 sequencer.
Name Strand Sequence
----------- ------- --------------------------
GJA3 EXON1A Forward 5'-CTGCGATGCCTGTCCTGTGG-3'
Reverse 5'-TTGTCCTGCGGTGGCTCCTT-3'
GJA3 EXON1B Forward 5'-CGCCCACCCTCATCTACCT-3'
Reverse 5'-GTGGGAACCCGATGGCAAC-3'
GJA3 EXON1C Forward 5'-AGCTCAAGCAGGGCGTGACC-3'
Reverse 5'-CAAGGGCGGCTGGTGCATCT-3'
GJA3 EXON1D Forward 5'-CCCCGGCGCTCAAGGCTTAC-3'
Reverse 5'-AACCCTTGTCCCCGCCACCC-3'
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