Table 1 of
Hawse, Mol Vis 2003;
9:515-537.Table 1 of
Hawse, Mol Vis 2003;
9:515-537.
Table 1. Primers used for RT-PCR
The table lists the sequences, GenBank accession numbers, annealing temperatures, product lengths, and PCR cycle numbers for all gene-specific primers used in this study.
Annealing Product Cycle Accession
Gene Abreviation Primer sequence temperature length number number
------------------ ------------- ------------------------- ----------- ------- ------ ---------
Hsp27-1 Hsp27-1 CGCGCTCAGCCGGCAACTCAG 64 419 27 XM_055937
Hsp27-1 Hsp27-1 AGGGGTGGGCATCCAGGCTAAGG 64 419 27 XM_055937
Hsp27-2 Hsp27-2 TCCTGACCCCCACACTCTACCA 61 421 27 NM_001541
Hsp27-2 Hsp27-2 GCTGCCTCCTCCTCTTCCTCTG 61 421 27 NM_001541
aA-crystallin aA CCACCTCGGCTCCCTCGTCCTAAG 64 492 25 NM_000394
aA-crystallin aA CCATGTCCCCAAGAGCGGCACTAC 64 492 25 NM_000394
RPL13a RPL13a GTATGCTGCCCCACAAAACCA 58 387 25 XM_027885
RPL13a RPL13a CAACGCATGAGGAATTAACAGTCTT 58 387 25 XM_027885
Metallothionein IF MTIF GCTTCTCTCTTGGAAAGTCC 55 226 30 M10943
Metallothionein IF MTIF GGCATCAGTCGCAGCAGCTG 55 226 30 M10943
Metallothionein IH MTIH GAACTCCAGTCTCACCTCGG 55 213 30 X64834
Metallothionein IH MTIH GACATCAGGCACAGCAGCTG 55 213 30 X64834
Metallothionein IG MTIG GCCTCTTCCCTTCTCGCTTG 55 234 30 XM_048213
Metallothionein IG MTIG GACATCAGGCGCAGCAGCTG 55 234 30 XM_048213
Glutathione GPX-1 GACCGACCCCAAGCTCATCACC 60 333 30 M21304
Peroxidase 1
Glutathione GPX-1 ATCAACAGGACCAGCACCCATCTC 60 333 30 M21304
Peroxidase 1
Na+/H+ Exchanger Na+/H+ Ex GCCATCTGTTTTGCGTTAGTGTTT 56 530 23 AF073299
II
Na+/H+ Exchanger Na+/H+ Ex GTTCGCTGACGGATTTGATAGAGA 56 530 23 AF073299
II
Serine/ Threonine S/T PK TGTTGGTGGGGATTTGCTTACTCT 57 449 23 NM_003607
Protein Kinase
Serine/ Threonine S/T PK CTTGGGCTGGAAACTGAAACCTCT 57 449 23 NM_003607
Protein Kinase
Na+/K+ ATPase Na+/K+ ATPase AAAGTACAAAGATTCAGCCCAGAG 52 419 23 BC000006
Na+/K+ ATPase Na+/K+ ATPase GGAGTTTGCCATAGTACGGATAAT 52 419 23 BC000006
Secreted Apoptosis SARP TTGTAATCCAGTCGGCTTGTTCTT 56 478 23 AF017987
Related Protein
Secreted Apoptosis SARP CTGGGCCTTTGCTGTCACTATTAC 56 478 23 AF017987
Related Protein
Pleiotrophin Ple. GTTCCCCGCCTTCCAGTCCA 60 430 23 M57399
Pleiotrophin Ple. TGCCCAGCCCACAGTCTCCA 60 430 23 M57399
E3-Ubiquitin UBE3-Lig CAGGGAATGGTTGTATCTCTTGTC 53 469 25 AY014180
Ligase
E3-Ubiquitin UBE3-Lig AATGCCTCGTAAAAATCTCCAGTT 53 469 25 AY014180
Ligase
aB-crystallin aB AGCCGCCTCTTTGACCAGTTCTTC 60 452 18 NM_001885
aB-crystallin aB GCGGTGACAGCAGGCTTCTCTTC 60 452 18 NM_001885
Catalase Cat TACCCCTCCTGGACTTTTTACATC 52 541 25 NM_001752
Catalase Cat CCTCATTCAGCACGTTCACATAGA 52 541 25 NM_001752
Metal-responsive MTF-1 GGGCCAGGACCTCAGCACAAT 59 445 25 XM_001412
Transcription
Factor 1
Metal-responsive MTF-1 AGAAGCCCCAGCAACAACAGAAAG 59 445 25 XM_001412
Transcription
Factor 1
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