Figure 2 of
Girao, Mol Vis 2003;
Figure 2. 7-keto stimulates differentiation and inhibits proliferation of LEC
Immunodetection of p57KIP2 (A), MIP26 (C), CP49 (D), γ-crystallin (E), and PCNA (G) in lens epithelial cells incubated with 7-keto. LEC were incubated with 100 ng/ml b-FGF (lane 2) for 10 days or 10 μg/ml 7-keto for 5 (lane 3) or 10 days (lane 4). The controls were incubated in the presence of ethanol 0.1% ethanol for 10 days (lane 1). Cell lysates were western blotted and probed with polyclonal antibodies directed against p57KIP2 (A), MIP26 (C), CP49 (D) or γ-crystallin (E), or probed with monoclonal antibodies directed against PCNA (G). Actin (B and F) was used as a loading control. Results are representative of at least three separate experiments. As positive controls for the presence of fiber specific proteins, lysates of entire bovine lenses (H) were western blotted and probed with polyclonal antibodies directed against MIP26 (line 1), CP49 (line 2) or γ-crystallin (line 3).