Table 3 of
Rogojina, Mol Vis 2003;
9:482-496.Table 3 of
Rogojina, Mol Vis 2003;
9:482-496.
Table 3. Atlas Glass fold changes relative to RT-PCR
Comparison of the results obtained on the Atlas Glass platform with real-time RT-PCR. Atlas and real-time RT-PCR results are shown as a fold change of the level of mRNA from the ARPE19 cell line relative to the level of that transcript in the D407 cell line. The changes of 5 of 15 genes analyzed (33%) were confirmed by real-time RT-PCR.
Fold Fold
Gene change change
number Title Unigene ID Atlas RT-PCR
------ ---------------------------------------- ---------- ------ ------
1 Actinin, alpha 1 Hs.119000 -1 2.4
2 Agouti (mouse) related protein Hs.104633 -1.2 3.2
3 Ras homolog, C Hs.179735 1.3 1.6
4 Activator of S phase kinase Hs.152759 -2.5 -1.5
5 ATPase, Cu++ transporting, alpha Hs.606 2.3 4.3
6 Beclin 1 Hs.12272 1.3 -2.2
7 CaM kinase, II gamma Hs.250857 -1.3 -1.2
8 CD81 antigen Hs.54457 1 1.9
9 Cyclin-dependent kinase 8 Hs.25283 1 1
10 Glyceraldehyde-3-phosphate dehydrogenase Hs.169476 -1.1 -2.1
11 Connexin 43 Hs.74471 -1.2 14.6
12 Integrin, alpha 3 Hs.265829 -1.5 1.9
13 Integrin, alpha V Hs.295726 -1.1 6.9
14 LDL receptor-related protein 8 Hs.54481 1.1 -1.3
15 Syntaxin 16 Hs.102178 1.1 -20
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