Table 2 of
Rogojina, Mol Vis 2003;
9:482-496.Table 2 of
Rogojina, Mol Vis 2003;
9:482-496.
Table 2. Affymetrix fold changes relative to RT-PCR
Comparison of the data obtained using the Affymetrix platform (HG-U95Av2 chips) with the results from real-time RT-PCR experiments. Affymetrix and real-time RT-PCR results are shown as a fold change of the level of mRNA from the ARPE19 cell line relative to the level of that transcript in the D407 cell line. A total of 22 genes were characterized by real-time RT-PCR. Out of these 22 genes, 10 were represented by several probe sets on the Affymetrix HG-U95Av2 chip. Real-time RT-PCR confirmed 85% (29 of 34) of the probe sets examined on the Affymetrix U95Av2 chip.
Fold
Gene Affymetrix Fold Change Change
number Title ProbeSet ID Affymetrix RT-PCR
------ ---------------------------------------- ----------- ----------- ------
1 Actinin, alpha 1 39329_at 3.3 2.4
Actinin, alpha 1 39330_s_at 3.1
2 Agouti (mouse) related protein 35068_at 1.2 3.2
3 Arg/Abl-interacting protein 39295_s_at -7.8 -1.1
4 Ras homolog, C 1395_at 1.3 1.6
5 Activator of S phase kinase 31865_at -4.8 -1.5
6 ATPase, Cu++ transporting, alpha 36523_at -1.6 4.3
7 Beclin 1 39378_at 1.6 -2.2
8 CaM kinase,II gamma 31670_s_at -1.5 -1.2
CaM kinase,II gamma 32104_i_at -1.2
CaM kinase,II gamma 32105_f_at -2.9
9 CD81 antigen 35282_r_at 1.6 1.9
10 Cyclin-dependent kinase 8 1189_at 1.1 1
Cyclin-dependent kinase 8 35140_at -1
11 Glyceraldehyde-3-phosphate dehydrogenase 39482_at -2.3 -2.1
12 Connexin 43 2018_at 1.5 14.6
Connexin 43 32531_at 4.1
13 Integrin, alpha 2 1978_at -2.8 -1.4
Integrin, alpha 2 41481_at -7.4
14 Integrin, alpha 3 884_at 3.5 1.9
Integrin, alpha 3 885_g_at 4.6
15 Integrin, alpha 4 2061_at 2.6 7.1
Integrin, alpha 4 35731_at 5
16 Integrin, alpha 5 39753_at -2 -2.5
17 Integrin, alpha V 257_at 1.7 6.9
Integrin, alpha V 39071_at 2.5
18 Integrin, beta 1 2055_s_at 3.2 1.1
Integrin, beta 1 33161_at 1.1
Integrin, beta 1 39483_s_at 1.2
19 LDL receptor-related protein 8 32398_s_at -2.9 -1.3
20 Syntaxin 16 39523_at -4.4 -20
Syntaxin 16 41121_at -1.1
21 ESTs integrin alpha 1 37484_at -1.3 -4.6
22 ESTs MHC class1 related sequence B 35937_at -6.4 -7.7
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