Figure 5 of
Proulx, Mol Vis 2003;
Figure 5. Cell density dependence of the α5 promoter activity in RPE cells
Sub-confluent (beige box; n=24), confluent (black box; n=30) and post confluent (white box; n=9) cultures of human RPE cells were transiently transfected with recombinant plasmids bearing the CAT reporter gene fused to various promoter segments from the human α5 gene. Levels of CAT activity for the transfected cells were normalized to the amount of hGH secreted into the culture media. Values are presented with standard deviation. An asterisk at the right side of the boxes indicates a promoter strength statistically different between the sub-confluent and confluent cells, or between the confluent and post-confluent cells, as indicated by the brackets (ANOVA; p<0.001). See text for individual p values.