Figure 2 of
Proulx, Mol Vis 2003;
9:473-481.
Figure 2. Proliferation assay
Primary human RPE cells were seeded at 1.5x105 cells/cm2 and remained in culture for either 2 days (confluent cells; A) or 2 weeks (post-confluent cells; B) before BrdU labeling (see Methods). As a negative control, the primary antibody was omitted (C). Scale bar represents 100 μm.