Table 1 of
Powell, Mol Vis 2003;
9:460-464.Table 1 of
Powell, Mol Vis 2003;
9:460-464.
Table 1. PCR conditions for OPA1 IVS8, TP53 IVS3, and TYRP1 exon 2
Primer sequences, PCR parameters and amplicon size data for the OPA1 (M13 primers), TP53, and TYRP1 genes.
PCR
Annealing product
Temperature PCR size
Gene Primer Oligonucleotide Sequence (°C) cycles (bp)
----- ------ ------------------------------------------------ ----------- ------ -------
OPA1 8FM13 5'-GGATAACAATTTCACACAGGTTTCTCTTGACACATCTGTTAT-3' 55 35 265
8RM13 5'-CACGACGTTGTAAAACGACTTAGGAAGCTAAGCTGCTTC-3'
TP53 I3F 5'-CTGAAAACAACGTTCTGGTA-3' 60 35 119/135
I3R 5'-AAGGGGGACTGTAGATGGGTG-3'
TYRP1 2F 5'-CGTGC TTCAGTCTTCTCTACA-3' 55 30 490
2R 5'-GCAGGACTTATGAACTCATTC-3'
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