Table 1 of
Morin, Mol Vis 2003;
9:449-459.Table 1 of
Morin, Mol Vis 2003;
9:449-459.
Table 1. Oligonucleotides used in this study
The oligonucleotides used for RT-PCR, 5'-RACE, 3'-RACE, plasmid construction, and site-directed mutagenesis are listed. The abbreviated names are those found in the text with "S" for sense and "AS" for antisense.
Name of
oligonucleotide or
oligonucleotide pair Sequence Purpose
-------------------- ------------------------------------------------------ --------------------
Pγ-rod S #1 5'-ATGAACCTGGAGCCACCCAAG-3 RT-PCR and 3'-RACE
Pγ-rod AS #1 5'-GATGATGCCATACTGGGCCAG-3' RT-PCR and 5'-RACE
Pγ-rod S #2 5'-GTGTCTCTGCCAGCCTCACC-3' 3'-RACE
Pγ-rod AS #2 5'-GGTTATATCTGTTCCCAGGCC-3' 5'-RACE
Pγ-cone S 5'-CTCCAACTTCAAACCAGGGTC-3 RT-PCR
Pγ-cone AS 5'-TCAGATGATCCCAAACTGAGC-3' RT-PCR
Anchor-poly-dG 5'-CUACUACUACUAGGCCACGCGTCGACTAGTACGGGIIGGGIIGGGIIG-3' 5'-RACE
Poly-dT-(Eco-Not) 5'-TTCTAGAATTCAGCGGCCGC-(T)25-(A/G/C/T)(A/G/C/T)-3' 3'-RACE
Eco-Not 5'-TTCTAGAATTCAGCGGCCGCTT-3' 3'-RACE
EcoRI-Pγ-rod S 5'-AAAGAATTCATGAACCTGGAGCCACCCAAG-3' Construction of
pBTM116-Pγ
BamHI-Pγ-rod AS 5'-AAAGGATCCCTAGGTTATATCTGTTCCCAGGCC-3' Construction of
pBTM116-Pγ
NcoI-CIP4 S 5'-GATGCCATGGACCGAGTGCCGTCGGACAGC-3' Construction of
pACT2-CIP4
XhoI-CIP4 AS 5'-AAACTCGAGTCAGTTGAGTGTGACTCGGAGGTA-3' Construction of
pACT2-CIP4
pACT2 S 5'-CACAGTTGAAGTGAACTTGCGG-3' PCR of pACT2 inserts
PACT2 AS 5'-TATCTATTCGATGATGAAGATACC-3' PCR of pACT2 inserts
Pγ (P5A) 5'-ATGAACCTGGAGGCGCCCAAGAGTGAG-3' and Mutation of proline
5'-CTCACTCTTGGGCGCCTCCAGGTTCAT-3' 5 of Pγ-rod to
alanine
Pγ (P20A) 5'-CGGGTGATGGGAGGCGCCGTCACCCCCAGG-3' and Mutation of proline
5'-CCTGGGGGTGACGGCGCCTCCCATCACCCG-3' 20 of Pγ-rod to
alanine
Pγ (P23A) 5'-GTGATGGGAGGGCCCGTCACCGCCAGGAAAGGACCA-3' and Mutation of proline
5'-TGGTCCTTTCCTGGCGGTGACGGGCCCTCCCATCAC-3' 23 of Pγ-rod to
alanine
Pγ (P27A) 5'-CCCAGGAAAGGAGCTCCTAAATTTAAG-3' and Mutation of proline
5'-CTTAAATTTAGGAGCTCCTTTCCTGGG-3' 27 of Pγ-rod to
alanine
Pγ (P20,23A) 5'-CGGGTGATGGGAGGCGCCGTCACCGCCAGGAAAGGACCACCT-3' Mutation of prolines
and 5'-AGGTGGTCCTTTCCTGGCGGTGACGGCGCCTCCCATCACCCG-3' 20 and 23 of Pγ-rod
to alanines
Pγ (P23,27A) 5'-GGGCCGGTCACCGCCAGGAAAGGAGCTCCTAAATTTAAG-3' and Mutation of prolines
5'-CTTAAATTTAGGAGCTCCTTTCCTGGCGGTGACCGGCCC-3' 23 and 27 of Pγ-rod
to alanines
Pγ (P42A) 5'-TTCAAGAGCAAGGCGCCCAAGAAAGGC-3' and Mutation of proline
5'-GCCTTTCTTGGGCGCCTTGCTCTTGAA-3' 42 of Pγ-rod to
alanine
FBP17 (W94A) 5'-GACAAAGGCGATGGCGCCACACGCATCCGCAGA-3' and Mutation of
5'-TCTGCGGATGCGTGTGGCGCCATCGCCTTTGTC-3' tryptophane 94 of
FBP17 to alanine
FBP17 (P108L) 5'-GAGGAGGGTTACGTACTCACTTCCTACGTCGAA-3' and Mutation of proline
5'-TTCGACGTAGGAAGTGAGTACGTAACCCTCCTC-3' 108 of FBP17 to
leucine
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