Figure 1 of
Morin, Mol Vis 2003;
Figure 1. Two-hybrid assay and in vitro interaction of Pγ-rod with the SH3 domain of FBP17
A: L40 yeast expressing the the Pγ-rod bait (as lexADBD fusion) were transformed with each of the two pACT2 clones (Number 10 and Number 14) isolated in the two hybrid screen and found to encode the SH3 domain of FBP17 (as gal4AD fusion). Negative controls were performed in parallel using L40 yeast expressing the neutral bait ERG19 (ERG). Transformants were plated as triplicate drops on medium lacking histidine. B: Glutathione-sepharose beads conjugated either to GST alone (GST) or to GST-Pγ-rod (GST-Pγ) were incubated with 35S-labeled FBP17 SH3 domain (starting material, SM). After washes, material specifically bound to the beads was eluted with Laemmli sample buffer and analyzed by SDS-PAGE and autoradiography.