Table 2 of
Xi, Mol Vis 2003;
9:410-419.Table 2 of
Xi, Mol Vis 2003;
9:410-419.
Table 2. Retinal crystallin transcript levels relative to Hprt
Quantitative analysis of crystallin transcripts in the retina by qRT-PCR. Hprt expression was used to normalize the expression levels. For each crystallin transcript, triplicate reactions were performed on four different total RNA samples. The hypothetical value of 2cycle threshold difference (100% reaction efficiency assumed) was used in calculating the fold difference from Hprt.
Mean difference of
cycle threshold from Fold difference from
Gene Hprt (±SEM) Hprt (Range)
----------------------- -------------------- --------------------
Alpha-A-Crystallin 2.76 ± 0.45 5.4 / 8.1
Alpha-A-INS-Crystallin 0.33 ± 0.27 0.1 / 2.5
Alpha-B-Crystallin -12.09 ± 0.29 -4355.3 / -4352.8
Alpha-A-Crystallin-nov1 -6.15 ± 0.44 -72.2 / -69.5
Beta-A1-Crystallin 0.86 ± 0.36 0.5 / 3.1
Beta-A2-Crystallin 1.40 ± 0.14 1.5 / 3.7
Beta-A3-Crystallin 2.01 ± 0.32 2.8 / 5.3
Beta-A4-Crystallin 0.35 ± 0.37 0 / 2.6
Beta-B1-Crystallin -3.03 ± 0.26 -9.4 / -7
Beta-B2-Crystallin 0.39 ± 1.18 -1 / 3.6
Beta-B3-Crystallin -1.26 ± 0.71 -4 / -0.8
Gamma-A-Crystallin -5.80 ± 1.07 -58 / -53.8
Gamma-C-Crystallin 0.71 ± 1.17 -0.6 / 3.9
Gamma-D-Crystallin 0.86 ± 1.15 -0.4 / 4.0
Gamma-E-Crystallin -5.28 ± 2.65 -45.1 / -32.5
Gamma-F-Crystallin -6.17 ± 2.22 -76.5 / -67.2
Gamma-S-Crystallin -10.52 ± 0.39 -1470.7 / -1468
Mu-Crystallin -7.47 ± 0.70 -178.8 / -175.5
Zeta-Crystallin -5.83 ± 0.15 -58 / 55.8
Lambda-Crystallin -8.30 ± 0.40 -317.4 / -314.7
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