Figure 4 of
Xi, Mol Vis 2003;
Figure 4. Expression of β-crystallin in mouse retinas
Expression of β-crystallin in adult mouse retinas by immunoblotting and immunofluorescence. Immunoblot analysis. (A) Cell lysates were prepared from 12.5 week old retinas and analyzed by SDS-PAGE and immunoblotting. A 20 μg aliquot of the retinal protein was applied to the gel. A monoclonal antibody against the bovine βH-crystallin fraction was used. Lanes are: left and middle, mouse retinas of two different animals; right, bovine lens βH-crystallin (1 μg). Note the significant variability of expression of β-crystallin in retina derived from two animals of the same litter. Immunofluorescence. (B) β-crystallin (red) was localized using a monoclonal antibody against bovine βH-crystallin fraction. Note that β-crystallin was distributed in the ganglion cell layer, inner photoreceptor layer, and outer nuclear layer. Note also the prominent punctate staining of β-crystallin in the photoreceptor inner segments. Cellular morphology was visualized with differential interference contrast. A DIC image of a normal retina is shown in (C). Pre-adsorption of the primary antibody with bovine βH-crystallin protein (negative control) showed no detectable β-crystallin immunofluorescence (D) in the retina shown in (C). Bar represents 13 μm (B-D).