Figure 3 of
Xi, Mol Vis 2003;
Figure 3. Expression of αB-crystallin in mouse retinas
Expression of αB-crystallin in adult mouse retinas by immunoblotting and immunofluorescence. Immunoblot analysis. (A) Cell lysates were prepared from 12.5 week old retinas and analyzed by SDS-PAGE and immunoblotting. A 20 μg aliquot of the retinal protein was applied to the gel. A polyclonal antibody against bovine αB-crystallin was used. Lanes are: left and middle, mouse retinas of two different animals; right, human lens αB-crystallin (0.1 μg). Note the significant variability of expression of αB-crystallin in retina derived from two animals of the same litter. Immunofluorescence. (B) αB-crystallin (red) was localized using an antibody to αB-crystallin. Cellular morphology was visualized with differential interference contrast. Note that αB-crystallin was distributed in the ganglion cell layer, inner photoreceptor layer, and outer nuclear layer (B). Note also the punctate staining of αB in the photoreceptor inner segments. A DIC image of an αB-/- retina (negative control) is shown in (C). In the αB-/- retina, there was no detectable αB-crystallin immunofluorescence (D). Bar represents 13 μm (B-D).