Table 2 of
O'Brien, Mol Vis 2003;
9:401-409.Table 2 of
O'Brien, Mol Vis 2003;
9:401-409.
Table 2. Summary of immunocytochemical (ICC) and RT-PCR data
At Fwk 9-10, ICC detected the expression of IRBP and TULP, while the corresponding PCR results were negative. Actin, Crx, and PDEB were detected at Fwk 10 by RT-PCR. By Fwk 12, ICC demonstrated labeling for Nrl and S opsin. RT-PCR at Fwk 12 detected TULP, IRBP, Nrl, and Crx, but not S opsin. The ICC and PCR data were consistent after Fwk 14 when all molecules investigated were expressed at high levels. In the table, "N/A" indicates not tested, "+" indicates expression was detected, and "-" indicates expression was not detected.
Fwk 9-10 Fwk 11-13 Fwk 14-16 Fwk 17-19
--------- --------- --------- ---------
ICC PCR ICC PCR ICC PCR ICC PCR
--------- --- --- --- --- --- --- --- ---
Actin N/A + N/A + N/A + N/A +
CRX N/A + N/A + N/A + N/A +
PDEB N/A + N/A + N/A + N/A +
IRBP + - + + + + + +
TULP + - + + + + + +
NRL + - + + + + + +
S opsin - - + - + + + +
L/M opsin - - - - + + + +
Rod opsin - - - - + + + +
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