Table 1 of
Wride, Mol Vis 2003;
9:360-396.Table 1 of
Wride, Mol Vis 2003;
9:360-396.
Table 1. PCR primers, conditions, and product sizes used here in RT-PCR reactions
PCR primers were designed using Primer3 on consensus sequences generated by cap3 using EST sequences in the database, and, where they existed, full-length reference sequences in the database, along with the sequences we generated. Information pertaining to the primers designed for genes in the various categories identified in our analysis is presented
PCR
EST Annealing Number product
accession Gene PCR Primers (F is forward temperature of size
number description and R is reverse) (°C) cycles (bp)
--------- ------------- --------------------------- ----------- ------ -------
Genes Preferentially Expressed in the Lens
Compared to Non Lens Samples
AI426509 Lenep F:TCGCCTACATCCTTCTTTGTTG 60 26 270
R:GAGCAAGGTTCGTGGTTCTTTC
AI323353 Mip F:TTCTATGTCTTCTTTGGGCTTGG 59 28 397
R:TCCGCCTCTCGTCGTATGTAG
AI325927 CryBA1 F:GCTCCTGCCCAAATGTCTCTG 64.5 23 200
R:GATGGGTCGGAAGGACATGAG
AI447387 Cerk F:TGCATACCAATTAGGCAACAGTC 58 27 213
R:TGCCACTTTCTAATCTTGAAGCC
AI451495 EST F:GTGGCTGATCTGGTTAGGCTTC 63 27 271
R:GGCAGGCACTCTGTTCTTCAC
AI528741 Col4a1 F:GCGCACTTGGTTTTCGTCTC 63 23 210
R:ACTCCACGCAGAGCAGAAGC
AI322438 Loxl F:TGCTGGAGCCACCTTACTTG 28 60 231
R:CCAGGCACTTCTCCTCTGCT
AI323855 Cd24a F:GGAACTACCAGGCGTTGCTT 62 27 231
R:TTCAGTGGTGGCTGGACTTG
AI448497 Dri2 F:AGAATCAACGGCAGGGAAGA 60 30 243
R:CCGGGATGAGGTAGGACTTG
Genes Up-Regulated in the 7 Day old Lens
Compared to Adult and Newborn Lens
AI452130 Wrn F:GTGGTATTCTCTGTGGCAGTGG 64 29 358
R:AAGCCCTCAGGTCAAGGAAAG
AI448302 EST F:TTCATAGCAAGTTTCAGGGCAG 61 28 220
R:TTGCCAGCTCAAGACAGAGAAG
AI428498 D15Ertd747e F:CAAGTTCAAAGGTCCCAAGAGG 61 28 310
R:CACCTTCAGAGTTTGGTGCAAG
AI448473 5033405M01Rik F:GTTAGGGAAGGAAATGAGCCAG 61 28 329
R:CTGCTTCACACAAGCCATCAG
AI452115 ESTs F:AAGAGAACCAGCCTGACGAGAC 61 28 298
R:AACTCTGCTTAGGAAAGGCCAG
AI465209 Fmod F:CCAGTAAGCATCTCCACAGGG 61 28 362
R:ATCAGAGGAGGGAAGTGTCCAG
AI450802 Rik F:TTCGTGATGGTCCGTAGTGG 61 30 278
R:GTTCTGGAAATGCCGTGATG
AI449772 Rik F:CCCGTGAGTGTTCTCATGCT 61 30 217
R:CAGCCCAAAGCAAACAGACTT
AI451673 Gtpbp2 F:TAGCCCTCTGCCCTTTCCTT 62.5 35 240
R:TATGTGGGTGCTGGGAACTG
Apoptosis Related Genes
AI323543 Pea15 F:GGGTGGGAGAAAGGAACTCT 62 27 206
R:AGGCTAGGAGCAACCTCACA
AI426523 Cradd F:GCTGAACCGGACTTTTCTCA 62 27 197
R:GGTAGGGTGGGTGTTGTGTT
AI326808 Gilz F:GGTGGTTCTGCGGTGTAAGT 62 27 204
R:CTGCTCTTTGTCAGGGGTCT
AI661160 Api5 F:CCTCAGTTTGCCACAGGAGA 62 27 235
R:CATCTGGGAGCGTCTTGAGTT
AI323465 Fsp27 F:GGCTGGTCACAGGAGGACTT 62 40 253
R:CTGCTCGCTTGGTTGTCTTG
AI327398 Ltb F:GGAGGCGAGCCAAGAAGAAG 61 42 278
R:ATAACGACCCGTACCCGATG
AI326797 Casp6 F:GCCGATTGCTTCATCTGTGT 60 30 246
R:ATCCACCTGGGTCACGTTGT
AI449135 Pmaip1 F:AAGCAATCAAGGGCTGAAGG 60 30 232
R:CGCTTTACGGTGGGAAAGAA
AI425932 Tiaf1 F:AACTGGCGTTTGATGACTTGG 61 32 344
R:ATTCTGGGTGGCTGGGTTCT
AI662639 Stambp F:TGTGTGCTGTGGTATGTGTGTTC 58 32 307
R:GCTTGGTGTGATTTGGTCATTT
Hemoglobins
Hba-a1 F:CCACCACCAAGACCTACTTTCC 58 32 282
R:AGGCAAGGAATTTGTCCAGAGA
Hba-X F:GCGGTTAAGAGCATCGACAAC 61 37 212
R:TTCTCAGTCAGGATAGAAGACAGGA
AI323916 Hbb-b1 F:ACCTGACTGATGCTGAGAAGG 61 32 244
R:CCCTTGACGCTGTCCAAGTG
Hbb-b2 F:CACCTGACTGATGCTGAGAAGT 61 33 245
R:CCTGTGGGCAAAGGTGAACC
Hbb-Y F:TTACTGCTGAGGAAAAGACCCTC 61 37 396
R:AAGTGACTAGCCAAAACAATCA
Selected Uncharacterized Genes
AI414484 0710008A13Rik F:ACTGGGTGAGAAACAGAAACCC 62 27 360
R:CACCCTCGAAGACCTCAGAAAG
AI426191 1810013D10Rik F:CTCGCTCCCTATTCACTTCCAC 62 27 233
R:AGGGTGAACACAAGCAACCTG
AI425997 ESTs F:CCTGAAAGACATCCACAAGACC 62 27 215
R:CCCTGTAAGTAAACGGCTGTCC
AI426997 ESTs F:GAGCAACTGAGGAAGACACTGG 60 27 207
R:TCCAACCAAACAAGCAATGAAG
AI451475 3010021M21Rik F:AACTCAGAAATCCACCTGCCTC 63 27 232
R:CTCTATTCAGGTATTAACCTTGCC
AI464327 EST F:ATGTTGGTCAGGGAGGAATTTG 61 33 241
R:AGGTGTGTGCCATCACTTTCTG
AI465543 4921516M08Rik F:TCACTCAGTCCACATAGTAGGAACAC 62 27 112
R:GAGATTGTGTAAGGTTTCTCACGG
AI661325 4921518A06Rik F:GCCTGACTCTCTGAATAATCTGGTATC 62 30 210
R:CTCTTGAAAATGTGTTTGGCACAG
AI323902 3100002B05Rik F:GGGGCTGAAGAGGAGTGAGC 62 28 219
R:TTTGCAGAAGTCGGCTGTTC
AI427110 2810455F06Rik F:TGTCACGCCCTTACTGGATG 62 27 247
R:CGTCTGTCCTTCCTTTGCTG
AI427165 C130058G02Rik F:GGGATGTTGTTCACTGCTGGT 28 60 261
R:TGAGCTTGGTGTTTGGTGGA
AI428574 ESTs F:CATCTCTCCTATGTGCCTCCAA 62.5 35 251
R:TCCCTCTGCTCTCAGGGTCT
AI450717 ESTs F:TGAGACAAAGGGACATGGAGAC 35 62.5 275
R:GCAGTGGGAAGGGCTATCAA
AI413436 C130043L22Rik F:GCACGACTTTAATCCCAGCA 60 35 254
R:TGCCTCAGCCTCAACAAACA
AI414993 IMAGE:5372918 F:GTGTGCTGGCTGTGTGTGAG 62.5 35 300
R:CCAGGGCTTTCAGCTTCTTG
AI447897 ESTs F:TTCCTGACACTTTCCTTCACCTC 61 35 280
R:AGCATTGATTCTCTACTCTGTGTTC
AI465228 2310035O07Rik F:CAGGCGAGGGAGATGAGAGA 64.5 32 373
R:GTGTTGGAGGCAGGAGAAGG
Selected Known Genes
AI323675 Pctk3 F:AGGGTCCAAGGAATAAGAAGGG 60 29 335
R:CACGGAACATGAACTCAGGAAG
AI327085 Hspe1 F:GGGTCAGGAGGGAAAGGAAAG 63 23 204
R:AGTGGAATGGCAGCTTCACG
AI327378 Atp10a F:CTGCCATCAGACTCGGGAAC 61 35 199
R:ATGAAACCAAAGCCCTGCTG
AI894153 Cd34 F:CCCAGCAAACTCCAGCTTATG 62 33 204
R:TCCTGTAGGGCTCCAACTGC
AI894311 Stk23 F:CAGGCCACACAGTTCAGTGC 64.5 35 213
R:ACCCAGGAGGATGGAAGAGC
AI427005 GTPbp3 F:TCGAGACCAGTACCCTGACTGA 62 27 245
R:TCGCTCCCTCTTCTTCTTGG
AI661257 Ifi203 F:CCCATTTCCTGACTTGCTGA 62 27 250
R:GGGAACTTGTCCTCCATCCA
AI661038 Flot1 F:CTCACAGGCGTCAGCATCTC 62 35 227
R:CTGGCAATGGGCTAAGAAGG
AI661287 Tgfbi F:GCACCAAATTCCCGAAAGAC 59 35 257
R:TGGGCAGGGCCAATATAGAA
AI429625 Sca10 F:GCACGACTTTAATCCCAGCA 62 35 254
R:TGCCTCAGCCTCAACAAACA
AI413145 Sssca1 F:GTGTCCTGGGCTTCCACTTC 64.5 30 253
R:GGTCGTGGGTGGCTACAGAG
AI430766 Ppp1r12a F:CAGCTCAACAGGCCAAACAG 64.5 30 248
R:CTGGAGCCGTCCTCAGTGTC
Controls
Actin F:CGTGGGCCGCCCTAGGCACCA 63 21 250
R:TTGGCCTTAGGGTTCAGGGGG
GAPDH F:ACCACAGTCCATGCCATCAC 61 23 450
R:TCCACCACCCTGTTGCTGTA
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