Figure 2 of
Girao, Mol Vis 2003;
Figure 2. Effect of proteasome inhibitors on the half life of phosphorylated Cx43
LEC were pulse-labeled with 32P for three h either in the absence (A) or presence of 40 μM MG132 (B) or 10 mM NH4Cl (C). During the last 30 min of incubation cells were exposed to 50 ng/ml TPA. The cells were chased for 1 and 3 h with non-labeled medium and Cx43 was immunoprecipitated using anti-Cx43 antibodies. Immunoprecipitated proteins were separated by SDS-PAGE and autoradiographed. D: Quantification of the amount of 32P labeled Cx43 at different times, as determined by the PhosphorImage analyser, Storm 860. Data represents the average of three independent experiments.