Figure 3 of
Chen, Mol Vis 2003;
Figure 3. Concentration and time dependent RA down-regulation of RPE65 protein
Immunoblot analysis of HEK293 cell extracts from cells treated with various concentrations of RA for the indicated times. A: Immunoblot using anti-RPE65 polyclonal antibody of cell extracts on samples treated with RA at 10-9-10-5 M. B: Panel A blot reprobed with anti-GAPDH monoclonal antibody to confirm equivalent loading. C: Immunoblot using anti-RPE65 polyclonal antibody on cell extracts of samples treated with RA (10-6 M) for 24, 48, or 72 h. D: Panel C blot reprobed with anti-GAPDH monoclonal antibody.