Figure 2. Rac GTPase activation induced by different growth factors in lens epithelial cells

Various growth factors tested in this study exert a definite activation of Rac GTPase in lens epithelial cells as shown in B, however with mean values of three experiments only EGF and FGF confirmed significant activation as compared to untreated cells. A representative western blot of Rac GTPase activation induced by different growth factors is shown in A. GTPγS and GDP treatment served as positive and negative controls, respectively, for the pull-down assays as in the case of Rho GTPase activation assay.