Figure 6. IRF-1 suppresses, whereas IRF-2 enhances transcription of the mimecan promoter

Bovine corneal keratocytes and T-47D cells were co-tranfected with wild-type construct (p-1314/-153 or p-1314/-1146) and either IRF-1, IRF-2, USF-1 and A-USF expression plasmids alone or in combinations. Cell lysates were analysed for luciferase and β-galactosidase activities 24 h after transfection. The results are presented as the means with standard error bars from three separate transfections performed in duplicate.