Figure 1. Detection of D-β-Asp-containing protein by western blot analysis using anti-peptide 3R antibody

SDS-PAGE (15%) and western blot analysis of protein (7 μg protein loaded) from the lens-derived cell lines were performed. A: Lane 1 contains protein from αTN4-1 cells, lane 2 contains protein from N/N1003A cells, lane 3 contains protein from HH cells, and lane 4 contains α-crystallin from the lenses of elderly donors. B: Immunoprecipitation of N/N1003A cell lysate with anti-peptide 3R antibody. Lysate of N/N1003A cells (containing about 0.2 mg total protein) was incubated with anti-peptide 3R antibody and immunoprecipitated protein was analyzed by SDS-PAGE. The gel was stained with Coomassie brilliant blue. Lane 1 contains immunoprecipitated protein and lane 2 contains total cell lysate.