Figure 4. Capillary formation of REC on Matrigel

The REC from wild type (A and C) and TSP1^-/- (B and D) were plated on Matrigel as described in Methods. After 7 h of incubation, wild type REC formed well-organized capillary-like structures (A), while TSP1^-/- REC ability to organize was severely compromised (B). The effects of PMA on capillary morphogenesis were minimal (C and D). These experiments were repeated twice with two-different strains of REC with similar results.