Figure 3 of
Su, Mol Vis 2003;
Figure 3. Western blot analysis of conditioned medium and cell lysates prepared from REC
The levels of TSP1 and fibronectin were analyzed by western blot analysis of serum free-conditioned medium prepared from REC as described in Methods. Note the absence of TSP1 in TSP1-/- REC. The TSP1-/- REC express more fibronectin, especially cell-associated fibronectin, compared to wild type REC. The blot was also incubated with anti-β-catenin as a loading control for intercellular protein levels.