Table 1 of
Bowne, Mol Vis 2003;
9:129-137.Table 1 of
Bowne, Mol Vis 2003;
9:129-137.
Table 1. PCR primers and restrictions enzymes used to analyze RP1L1
Amplimer 4N PCR product was sequenced using the following nested sequencing primers: 5'-CCT TCA CCC TGG AGG ACG-3'; 5'-GGC TGG ACC TCC CAT TC-3'. Amplimer 4O primers incorporated M13 sequences into the PCR products. M13 sequencing primers were then used to sequence this amplimer.
Annealing Product Restriction
Amplimer Primer sequences 5'-3' temperature size (bp) enzyme
-------- ------------------------------------------- ----------- --------- -----------
2A GCCAATCCCCCAAGCTG 60 °C 321 BamHI
GGGTGTGGTGACAGAGCG
2B CGTGCCTCTCTCCTTTGG 60 °C 444 HinfI
AGGTCTAAAGAACCTTTTCAAGG
3 TGGTGAGACTGGATCCTTCC 60 °C 292 HpaII
CAGCCCTACTGAACCACCAT
4A CTGTTTTATTCCTTTATCCTGACGC 54 °C 417 AluI
CTACCTCCCCCAGAACGG
4B GCTTCCACCTGGTCGGCG 54 °C 386 EaeI
GGCTGGGCTGGCACTGTC
4C GGAAGAGGTGGGGACTGG 62 °C 411 DdeI
TTGCCTTGCCTGGACAGC
4D AGCGAATGGGGTGGGCGG 54 °C 404 MboII
GAGTCCAGTGGGCTGTGG
4E TCCCAGGCATTCTCACTACC 54 °C 362 BsaI
AGCAGGAGTCGGATGTGTG
4F CGGCCCCATACCTCCCCAC 60 °C 229 None
TGAGCAGCAGTGGCTTCG
4G GCCTCAGCCCCTCCTCACC 60 °C 392 RsaI
TCCTCAAGGTCTTCTCCTCG
4H CAGTGCCAGCCAGGGTGC 54 °C 207 None
GTGGTCTCGTCCGCCAAC
4I ATGGCTGGACAACATTCCA 54 °C 393 StuI
ATCAGCGCCCTCATGATCT
4J CCGGAGCAGACAGAGAGG 54 °C 410 BsaI
CGTGAAGTTCTCCGTCATGG
4K GATGTGACGTTGGGGAAGAC 54 °C 401 MslI
AGCTAACTGCTCCAGGTTCG
4L AGAGACAGTGAGGAGCAGAGG 60 °C Variable None
TCTCCTTGCAGTCCTCCTTC
4M GAGGGGGTGCAGTTAGAGG 62 °C 874 None
CCTCGCAGGGACAGAACTC
4N CTCTCCTTCACCCTGGAGG 65 °C 829 None
CTTCTGACTCTGGCTGGACC
4O GTAAAACGACGGCCAGTCCCCAGAGGCAGAAGGAG 69 °C 861 None
CCTTTGTCGATACTGGTACTGCCTCTACACCTTCTGACTCAGG
4P GAAGGGGAGGCCCAGAAG 62 °C 919 None
CCAAGCTCGTGATTGTTTTC
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