Figure 4 of
Srivastava, Mol Vis 2003;
Figure 4. Western blot analysis of TCA soluble polypeptides from normal lenses
Western blot analysis of 2D-gel separated TCA soluble polypeptides from WS proteins of normal lenses. Two antibodies, designated as anti-human αA-crystallin N-terminal peptide (residues number 1-9) and anti-human αA-crystallin C-terminal peptide (residues number 165-173), were used. Three 4-kDa spots (numbered 1, 2, and 3) exhibited immunoreaction only with the anti-αA-crystallin C-terminal peptide. Partial N-terminal sequence analysis of all three spots showed an identical N-terminal sequence of GMLTF, suggesting a cleavage at D136-G137 in αA-crystallin.