Figure 1. Effects of SPARC protein on HRPE cell proliferation

MTS assays were conducted on cultured HRPE cells. Four days after cells were seeded into the wells of 96 well plates with the test media, MTS reagent was added and the absorbance of the formazan produced in each well was read at 525 nm using a microtiter plate reader. Test media constituents were as noted in figure. The results are expressed as a percentage of the positive control, which was F10 medium with 20% fetal calf serum (FCS). A significant decrease in cellular dehydrogenase activity (absorbance) was observed with 1 and 10 μg/ml SPARC, represented as a decrease in percent proliferation (*p<0.01). Error bars represent the standard error of the mean.