Figure 3. cGMP-PDE activity

cGMP-PDE activity of extracts from pCIS2- and pC57-transfected HEK293 (A) and CHO (B) cells. The assay was carried out using 100 μM cGMP as substrate at 37 °C and ~80 μg of total protein. Results are from typical experiments conducted in triplicate (±SD). C: Inhibition by the ROS-1 monoclonal antibody of cGMP-PDE activity in homogenates from pCIS2- and pNC57-transfected CHO cells. Cell extracts (80 μg protein) were incubated with a 1:20 dilution of ROS-1 antiserum at 4 °C for 3 h, then assayed for cGMP-PDE activity with 50 μM cGMP as substrate. Activities are presented as percent of that of control extracts which were incubated without the ROS-1 antiserum.