Figure 2 of
Tasheva, Mol Vis 2003;
9:1-9.
Figure 2. Evidence for p53 dependent induction of human mimecan transcription
A: Schematic representation of the 5' region of the human mimecan gene and position of the primers used for PCR. B: Increase in mimecan gene expression in U2-OS cells or U2-OS cells transfected with p53 expression plasmid, following UV irradiation. Cells were treated with 20 J/m2 of UV radiation and harvested 24 h after UV exposure, or transfected with the wild type p53 and 24 h later treated with 20 J/m2 of UV radiation. RNA was isolated 24 h after UV treatment and subjected to RT-PCR for human mimecan. GAPDH controls were included to verify integrity of RNA and equal loading. C: Increase in mimecan gene expression in Saos-2 cells transfected with p53 expression plasmid following UV irradiation. The letter "M" designates the Hi-Lo DNA marker (Minnesota Molecular)