Figure 3 of
Wagner, Mol Vis 2002;
Figure 3. Phosphorylation of Cx43 by PKCg
A: Cx43 was co-immunoprecipitated with anti-PKCg antisera from cells overexpressing PKCg. Immunoprecipitated proteins were analyzed using 12.5% SDS-PAGE and western blot, then the membranes which contained the transferred proteins were probed with anti-phosphotyrosine (lane 1), anti-phosphoserine (lane 2), and anti-phosphothreonine (lane 3) antibodies. B: Western blots were probed with anti-Cx43 antibodies to determine that equal amounts of immunoprecipitated Cx43 was in each sample. All antisera were used at 1:1000 dilution.